APPLICATION
Biochemical / Biomedical
★ Clinical Biochemistry Analyzer ★
The advantages of choosing a spectrometer
● The world's only "multifunctional blood analyzer" with low blood volume, high accuracy, and competitive price, with OtO halogen lamp balance solution. The stray light calibration algorithm can achieve 300 -800nm measureable by OD 2.5 ultra-precision.● At present, the clinical analyzer with the most complete functions of a single model in the industry, is different from the filter-type optical platform. It uses OtO'S spectrometer as the core module to test NH3, T4, Bile acid, Phenobarbital, Cortisol, CRP and other special items.
Balanced light source-BL60 for Biochemical Test
● Make up the most needed light intensity in the 330 ~ 400nm band in the field of biochemical analysis
● The halogen lamp is used to strengthen the ultraviolet band and provide the most stable light intensity
● With SmartEngine-6, high signal-to-noise ratio (SNR = 500), to achieve the most accurate measurement
● Complete customized fixtures, integrated into the measurement platform in real-time
◆ Algorithms of Stray Light Calibration (SC)
OtO has a very powerful stray light calibration (SC) capability because of our strong expertise in optical design and software engineering. Level of stray light has been decreased to only 0.01% after our software stray light calibration. In case of the measurement needs for low transmittance, low reflectance, high absorbance, weak light source and extremely short integration time, the calibration will work well. There is no need to spend any calculation time when measuring.
The great contribution to low transmittance
| Standard values of transmittance | Measured values before SC | Measured values after SC | |
|---|---|---|---|
| Stray Light Level: 0.12% | Stray Light Level: 0.05% | Stray Light Level: 0.01% | |
| 90.00% | 90.01% | 90.00% | 90.00% |
| 10.00% | 10.11% | 10.04% | 10.01% |
| 1.00% | 1.12% | 1.05% | 1.01% |
| 0.10% | 0.22% | 0.15% | 0.11% |
◆ Recommended Models :
| Application | Model Name | Wavelength Range (nm) | Slit (um) | Resolution | SNR | Exposure Time (ms) | Dynamic Range | Thermal Stability |
| Blood Analysis | SE-3 | 300- 850 | 50 | 1.9 nm | 330 | 0.2 | 3000 | 0.027 nm/℃ |
| HB-3 | 300- 850 | 25 | 2.3 nm | 330 | 0.21 - 10 | 3120 | 0.01 nm/℃ | |
| Fluorescence Detection | SE-3/ SE-8 | 340- 850 | 50 | 1.9 nm | 330/ 350 | 0.2 / 0.42 | 3000 / 2200 | 0.027 nm/℃ |
| DNA Analysis | HB-3/ HB-9 | 180- 850 | 50 | 1.9 nm | 330 | 0.21 - 10 | 3120 / 2920 | 0.01 nm/℃ |
| OCT Application | HB-3/ HB-8 | 790- 1090 | 25 | 0.6 nm | 330 | 0.21 - 10 | 3120 / 2200 | 0.01 nm/℃ |
| SE-3/ SE-8 | 790-1010 | 50 | 0.9 nm | 330/ 350 | 0.2 / 0.42 | 3000 / 2200 | 0.027 nm/℃ |
◆ The advantages of choosing a spectrometer for biochemical applications
By applying a spectrometer as optical core of a biochemical analyzer, one single module can be used to measure all wavelengths of reagents simultaneously. In addition, the build-in CPU and custom software can be used to complete calculation of parameters, such as linear equations, standard deviation, bias, etc., which can optimize the efficiency and accuracy of biochemical analyzer at the same time.
For example, the range of response wavelengths of biochemical test items is different (Table 1). When the optical module is based on various optical filters, the test items and range which are detected by a single sensor will be limited by the fixed wavelengths. In order to complete the analyzer’s functions, there should be additional optical filters, photodiodes, and calculation system. On the contrary, one spectrometer can obtain full-spectrum signals and provide the analyzer with unlimited expansion flexibility. Full-spectrum signals also serve as a standard for calibrating wavelength, intensity, linearity and stray light. In order to pursue high stability and high accuracy (to get FDA certification) of multifunctional biochemical devices, applying the spectrometer to be the core optical module is the best choice.
For example, the range of response wavelengths of biochemical test items is different (Table 1). When the optical module is based on various optical filters, the test items and range which are detected by a single sensor will be limited by the fixed wavelengths. In order to complete the analyzer’s functions, there should be additional optical filters, photodiodes, and calculation system. On the contrary, one spectrometer can obtain full-spectrum signals and provide the analyzer with unlimited expansion flexibility. Full-spectrum signals also serve as a standard for calibrating wavelength, intensity, linearity and stray light. In order to pursue high stability and high accuracy (to get FDA certification) of multifunctional biochemical devices, applying the spectrometer to be the core optical module is the best choice.
| Analyte | Methodology | Absorption wavelength |
| Glucose | Fluorescent spot test | 340nm |
| Protein | UV-VIS | 270-290nm / 200-225nm |
| Electrolyte (Chlorine ion) | Colorimetric test | 480nm |
| Uric acid | ELISA | 293nm |
| Bilirubin | Colorimetric test | 455nm / 575nm (Hemoglobin interference correction) |
| Triglyceride | UV-VIS | 232nm |
Table 1. Wavelength ranges of common biochemical test item
As the only spectrometer design and development leader in Taiwan, OtO Photonics has launched available spectrometers by applying conventional Czerny-Turner structure － Smart Engine TM (SE) series and Humming Bird TM (HB) series for biochemical applications.
Moreover, OTO Photonics has launched UM series (UM, Ultra Micro Modular Spectrometer) for portable device market by applying the patented MEMS (Micro-electro-mechanical Systems) waveguide and concave grating － The winner of 2018 National Invention and Creation “Golden Award" in Taiwan.
▲ Progress from conventional optical layout to OtO Photonics’ MEMS technology
In a MEMS spectrometer, the precision of waveguide dimensions is improved by at least 20 times, the yield rate is increased from 50% to 95%, and the cost is reduced to one tenth of conventional design. It makes UM series become the world's smallest (23mm*31mm*8.5mm) and highest resolution (5nm) spectrometer. OTO Photonics predicts that market share of portable analyzer will grow in the next few years.
Moreover, OTO Photonics has launched UM series (UM, Ultra Micro Modular Spectrometer) for portable device market by applying the patented MEMS (Micro-electro-mechanical Systems) waveguide and concave grating － The winner of 2018 National Invention and Creation “Golden Award" in Taiwan.
▲ Progress from conventional optical layout to OtO Photonics’ MEMS technology
In a MEMS spectrometer, the precision of waveguide dimensions is improved by at least 20 times, the yield rate is increased from 50% to 95%, and the cost is reduced to one tenth of conventional design. It makes UM series become the world's smallest (23mm*31mm*8.5mm) and highest resolution (5nm) spectrometer. OTO Photonics predicts that market share of portable analyzer will grow in the next few years.
★ Skin-OCT scanner ★
◆ Applying spectrometer as the optical core
In Taiwan, the Industrial Technology Research Institute (ITRI) have made a breakthrough –– the world's smallest and fastest OCT skin scanner.
By applying OtO Photonics modular spectrometers, SE2030 and HB2030, can scan 2000 points of tomographic data by handheld probe in a very short period of time. With the built in multitasking ARM9 CPU and 32MB memory of OtO spectrometer. It can process a large amount of spectral information in one second, and then obtain instant skin condition through the patented algorithm, and display the detection result with micron tomographic image.